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Image Search Results
Journal: Journal of Cerebral Blood Flow & Metabolism
Article Title: Microvascular plasticity in mouse stroke model recovery: Anatomy statistics, dynamics measured by longitudinal in vivo two-photon angiography, network vectorization
doi: 10.1177/0271678X241270465
Figure Lengend Snippet: Experimental Methods: (a) Mouse cortex is imaged in vivo through a cranial window using a tiling protocol to cover as large of a volume as possible in a single imaging session. The stitched image is vectorized using the SLAVV software for reconstruction, visualization, and statistical analysis. The vessel directions (as well as the borders of the 2PM images and the ROI borders) are color-coded with respect to their alignment with the imaging coordinate system: ( xyz <->CMY). (b) LSCI is used to orient the 2PM imaging session to reproducibly image the same (∼1 mm 3 ) volume longitudinally over several imaging sessions at two-week intervals and at a depth greater than 600 micrometers. Displayed is an orthographic projection in the (optical) z- axis of a tiled image volume of a healthy control subject. Lateral orthographic projections show the longitudinal imaging reproducibility and (c) the longitudinal experiment is repeated around a photothrombotic injury. The infarct appears to be contained to a (yellow) circle with a radius of approximately 1 mm in the LSCI post-stroke image. The x-projected 2PM image reveals that a (transparent cyan) spherical ROI beneath the surface approximates the shape of the infarct. Scale bars are 200 μm.
Article Snippet: 2PM volumetric angiographs were vectorized using the
Techniques: In Vivo, Imaging, Software, Control